[vc_row css=”.vc_custom_1459506798851{margin-bottom: 100px !important;}”][vc_column offset=”vc_col-lg-9 vc_col-md-9″][vc_single_image image=”2201″ img_size=”stm-image-1110×550-croped” css=”.vc_custom_1577796613605{margin-bottom: 45px !important;}”][vc_custom_heading text=”Plant Tissue Culture
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Sterilization techniques:
- Dry heat sterilization
- Moist heat sterilization
- Filtration
Preparation of media
- Nutritional component of the media
Invitro germination of seeds
- Surface sterilization of seed
- Invitro Plant regeneration technique
- Micropropagation
- Callus culture
- Embryo culture
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Preparation of explants
- Starter culture preparation
- Co cultivation of explants with the
- bacteria
- Selection media transfer.
Analysis of transgenic plants
- Extraction of DNA
- Agarose gel electrophoresis
- PCR
ELISA:
- Extraction of protein samples
- Processing of the samples for the
- detection of viral antigen.
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