Molecular Cloning
Preparation of nucleic acids
- Plant Genomic DNA extraction
- Plasmid DNA extraction
- Purification of gDNA & plasmid DNA
Agarose gel electrophoresis. Spectrophotometer, Dot spot
Preparation of target gene fragment:
- PCR techniques
- Agarose gel electrophoresis
- Gel elution
- Restriction digestion
- Dot spot quantification
- Ligation
- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
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