Molecular Cloning

2

Molecular Cloning

Preparation of nucleic acids
  • Plant Genomic DNA extraction
  • Plasmid DNA extraction
  • Purification of gDNA & plasmid DNA

Agarose gel electrophoresis. Spectrophotometer, Dot spot

Preparation of target gene fragment:
  • PCR techniques
  • Agarose gel electrophoresis
  • Gel elution
  • Restriction digestion
  • Dot spot quantification
  • Ligation
  • Preparation of nutrient broth
  • Starter culture preparation
  • Preparation of competent cells
  • Storage of competent cells
  • Preparation of nutrient broth
  • Starter culture preparation
  • Preparation of competent cells
  • Storage of competent cells
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