List of Courses - Hybrinomics

[vc_row css=”.vc_custom_1459506798851{margin-bottom: 100px !important;}”][vc_column offset=”vc_col-lg-9 vc_col-md-9″][vc_single_image image=”2161″ img_size=”stm-image-1110×550-croped” css=”.vc_custom_1577790280752{margin-bottom: 45px !important;}”][vc_custom_heading text=”List of Courses” font_container=”tag:h4|text_align:left|color:%23222222″ use_theme_fonts=”yes” css=”.vc_custom_1577855935370{margin-bottom: 31px !important;}”][vc_tta_accordion shape=”square” c_position=”right” active_section=”1″ css=”.vc_custom_1574145661399{margin-bottom: 34px !important;}”][vc_tta_section tab_id=”1452765420119-d4dfaf69-4fae” title=”Molecular Cloning”][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Recombine DNA Technology Duration: 15 days
Preparation of nucleic acids

Plant Genomic DNA extraction
Plasmid DNA extraction
Purification of gDNA & plasmid DNA

Agarose gel electrophoresis. Spectrophotometer, Dot spot
Preparation of target gene fragment:

PCR techniques
Agarose gel electrophoresis
Gel elution

Preparation of plasmid DNA & gene fragment for cloning:

Restriction digestion
Dot spot quantification
Ligation

Competent cell Preparation

Preparation of nutrient broth
Starter culture preparation
Preparation of competent cells
Storage of competent cells

Bacterial transformation

Preparation of LB agar plates
Transformation of recombinant DNA into E.coli cell
Plating technique for transformed culture
Transformation efficiency

Cell lysis and protein extraction

Separation of protein molecules:

SDS-PAGE (Poly Acrylamide Gel Electrophoresis)

Staining Techniques

Coomassie brilliant blue staining
Silver staining

Data Analysis

Estimation of molecular mass of protein of interest and relative abundance of unknown polypeptides in a complex mixture

Extraction of protein sample containing antigen
SDS-PAGE (poly Acrylamide gel electrophoresis)
Handling of nitrocellulose membrane
Transfer of protein bands from gel to nitrocellulose membrane

Immuno Assay:

Processing of nitrocellulose membrane for detection of antigen

Serial dilution techniques:

Preparation of sample material
Preparation of nutrient media
Plating the culture

RAPD
SSR
SCAR
CAPS

Preparation of nucleic acid:

Extraction of DNA
Purification of DNA

PCR Techniques:

Gene analysis
Genetic purity {Homozygosity & homogeneity}
Genetic diversity

Gel Electrophoresis Technique:

Agarose gel electrophoresis
Native PAGE
SDS – PAGE

Gel Staining Techniques:

Ethidium bromide staining (PAGE/Agarose)
Silver staining

Data Analysis:

Analysis of resistant & susceptible lines
NTysis software

Screening of transformed colonies:

Alpha complementation
Colony/Plasmid PCR

Dry heat sterilization
Moist heat sterilization
Filtration

Preparation of media

Nutritional component of the media
Invitro germination of seeds

Invitro Plant regeneration technique

Micropropagation
Callus culture
Embryo culture

Agrobacterium-mediated gene transformation into a plant

Preparation of explants
Starter culture preparation
Co cultivation of explants with the bacteria
Selection media transfer.

Analysis of transgenic plants

Extraction of DNA
Agarose gel electrophoresis
PCR

ELISA:

Extraction of protein samples
Processing of the samples for the detection of viral antigen.

Plant material
Soil sample
Peat

Identification of pathogens:

Microscope
Gram staining
Motility

[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Microbiology” tab_id=”1577860155918-cf641a77-600c”][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text][/vc_column_text][vc_column_text][/vc_column_text][vc_raw_html]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[/vc_raw_html][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Projects” tab_id=”1577860578426-d9642476-dc41″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]

Long duration projects Duration: 2-6 months
Project areas

- - 1. DNA finger printing
  - 2. Marker assisted trait selection
  - 3. Plant tissue culture
  - 4. Plant pathology
  - 5. Seed Quality Assurance