[vc_row css=”.vc_custom_1459506798851{margin-bottom: 100px !important;}”][vc_column offset=”vc_col-lg-9 vc_col-md-9″][vc_single_image image=”2161″ img_size=”stm-image-1110×550-croped” css=”.vc_custom_1577790280752{margin-bottom: 45px !important;}”][vc_custom_heading text=”List of Courses” font_container=”tag:h4|text_align:left|color:%23222222″ use_theme_fonts=”yes” css=”.vc_custom_1577855935370{margin-bottom: 31px !important;}”][vc_tta_accordion shape=”square” c_position=”right” active_section=”1″ css=”.vc_custom_1574145661399{margin-bottom: 34px !important;}”][vc_tta_section tab_id=”1452765420119-d4dfaf69-4fae” title=”Molecular Cloning”][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Recombine DNA Technology Duration: 15 days
Preparation of nucleic acids
- Plant Genomic DNA extraction
- Plasmid DNA extraction
- Purification of gDNA & plasmid DNA
Agarose gel electrophoresis. Spectrophotometer, Dot spot
Preparation of target gene fragment:
- PCR techniques
- Agarose gel electrophoresis
- Gel elution
Preparation of plasmid DNA & gene fragment for cloning:
- Restriction digestion
- Dot spot quantification
- Ligation
Competent cell Preparation
- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
Bacterial transformation
- Preparation of LB agar plates
- Transformation of recombinant DNA into E.coli cell
- Plating technique for transformed culture
- Transformation efficiency
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”SDS-Poly Acrylamide Gel Electrophoresis” tab_id=”1574146865006-b8f67bf9-f1d4″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Recombine DNA Technology Duration: 15 days
Estimation of protein:
- Cell lysis and protein extraction
Separation of protein molecules:
- SDS-PAGE (Poly Acrylamide Gel Electrophoresis)
Staining Techniques
- Coomassie brilliant blue staining
- Silver staining
Data Analysis
- Estimation of molecular mass of protein of interest and relative abundance of unknown polypeptides in a complex mixture
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Immunology” tab_id=”1574146864373-74aec471-8f15″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Immunology Duration: 15 days
Western blotting:
- Extraction of protein sample containing antigen
- SDS-PAGE (poly Acrylamide gel electrophoresis)
- Handling of nitrocellulose membrane
- Transfer of protein bands from gel to nitrocellulose membrane
Immuno Assay:
- Processing of nitrocellulose membrane for detection of antigen
Serial dilution techniques:
- Preparation of sample material
- Preparation of nutrient media
- Plating the culture
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”DNA Fingerprinting Technology” tab_id=”1574146863557-49506da1-f1e4″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in DNA fingerprinting Technology Duration: 45 days
Molecular markers:
- RAPD
- SSR
- SCAR
- CAPS
Preparation of nucleic acid:
- Extraction of DNA
- Purification of DNA
PCR Techniques:
- Gene analysis
- Genetic purity {Homozygosity & homogeneity}
- Genetic diversity
Gel Electrophoresis Technique:
- Agarose gel electrophoresis
- Native PAGE
- SDS – PAGE
Gel Staining Techniques:
- Ethidium bromide staining (PAGE/Agarose)
- Silver staining
Data Analysis:
- Analysis of resistant & susceptible lines
- NTysis software
Screening of transformed colonies:
- Alpha complementation
- Colony/Plasmid PCR
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Plant tissue Culture” tab_id=”1574146862399-3ce1d06c-74d9″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Plant tissue culture Duration: 15 days
Sterilization techniques:
- Dry heat sterilization
- Moist heat sterilization
- Filtration
Preparation of media
- Nutritional component of the media
- Invitro germination of seeds
Invitro Plant regeneration technique
- Micropropagation
- Callus culture
- Embryo culture
Agrobacterium-mediated gene transformation into a plant
- Preparation of explants
- Starter culture preparation
- Co cultivation of explants with the bacteria
- Selection media transfer.
Analysis of transgenic plants
- Extraction of DNA
- Agarose gel electrophoresis
- PCR
ELISA:
- Extraction of protein samples
- Processing of the samples for the detection of viral antigen.
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Plant Pathology” tab_id=”1577859952796-bee585eb-f35f”][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Training in Plant pathology Duration: 7 days
Detection of pathogens:
- Plant material
- Soil sample
- Peat
Identification of pathogens:
- Microscope
- Gram staining
- Motility
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Microbiology” tab_id=”1577860155918-cf641a77-600c”][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text][/vc_column_text][vc_column_text][/vc_column_text][vc_raw_html]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[/vc_raw_html][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:https%3A%2F%2Fwww.hybrinomics.com%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Projects” tab_id=”1577860578426-d9642476-dc41″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]
Long duration projects Duration: 2-6 months
Project areas
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- 1. DNA finger printing
- 2. Marker assisted trait selection
- 3. Plant tissue culture
- 4. Plant pathology
- 5. Seed Quality Assurance
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