List of Courses
Training in Recombine DNA Technology Duration: 15 days
Preparation of nucleic acids
- Plant Genomic DNA extraction
- Plasmid DNA extraction
- Purification of gDNA & plasmid DNA
Agarose gel electrophoresis. Spectrophotometer, Dot spot
Preparation of target gene fragment:
- PCR techniques
- Agarose gel electrophoresis
- Gel elution
Preparation of plasmid DNA & gene fragment for cloning:
- Restriction digestion
- Dot spot quantification
- Ligation
Competent cell Preparation
- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
Bacterial transformation
- Preparation of LB agar plates
- Transformation of recombinant DNA into E.coli cell
- Plating technique for transformed culture
- Transformation efficiency
Training in Recombine DNA Technology Duration: 15 days
Estimation of protein:
- Cell lysis and protein extraction
Separation of protein molecules:
- SDS-PAGE (Poly Acrylamide Gel Electrophoresis)
Staining Techniques
- Coomassie brilliant blue staining
- Silver staining
Data Analysis
- Estimation of molecular mass of protein of interest and relative abundance of unknown polypeptides in a complex mixture
Training in Immunology Duration: 15 days
Western blotting:
- Extraction of protein sample containing antigen
- SDS-PAGE (poly Acrylamide gel electrophoresis)
- Handling of nitrocellulose membrane
- Transfer of protein bands from gel to nitrocellulose membrane
Immuno Assay:
- Processing of nitrocellulose membrane for detection of antigen
Serial dilution techniques:
- Preparation of sample material
- Preparation of nutrient media
- Plating the culture
Training in DNA fingerprinting Technology Duration: 45 days
Molecular markers:
- RAPD
- SSR
- SCAR
- CAPS
Preparation of nucleic acid:
- Extraction of DNA
- Purification of DNA
PCR Techniques:
- Gene analysis
- Genetic purity {Homozygosity & homogeneity}
- Genetic diversity
Gel Electrophoresis Technique:
- Agarose gel electrophoresis
- Native PAGE
- SDS – PAGE
Gel Staining Techniques:
- Ethidium bromide staining (PAGE/Agarose)
- Silver staining
Data Analysis:
- Analysis of resistant & susceptible lines
- NTysis software
Screening of transformed colonies:
- Alpha complementation
- Colony/Plasmid PCR
Training in Plant tissue culture Duration: 15 days
Sterilization techniques:
- Dry heat sterilization
- Moist heat sterilization
- Filtration
Preparation of media
- Nutritional component of the media
- Invitro germination of seeds
Invitro Plant regeneration technique
- Micropropagation
- Callus culture
- Embryo culture
Agrobacterium-mediated gene transformation into a plant
- Preparation of explants
- Starter culture preparation
- Co cultivation of explants with the bacteria
- Selection media transfer.
Analysis of transgenic plants
- Extraction of DNA
- Agarose gel electrophoresis
- PCR
ELISA:
- Extraction of protein samples
- Processing of the samples for the detection of viral antigen.
Training in Plant pathology Duration: 7 days
Detection of pathogens:
- Plant material
- Soil sample
- Peat
Identification of pathogens:
- Microscope
- Gram staining
- Motility
- Dry heat sterilization
- Moist heat sterilization
- Filtration
- Solid media
- Liquid media
- Spread plate method
- Pour plate method
- Streak plate method
- Starter culture preparation
- Spectrophotometer
