Immunohistochemistry

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Immunohistochemistry Course List

Western Blotting:
  • Extraction of protein sample containing antigen
  • SDS-PAGE (poly Acrylamide gel electrophoresis)
  • Handling of nitrocellulose membrane
  • Transfer of protein bands from gel to nitrocellulose membrane

Processing of nitrocellulose membrane for detection of antigen

  • Preparation of sample material
  • Preparation of nutrient media
  • Plating the culture
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