Immunohistochemistry Course List
Western Blotting:
- Extraction of protein sample containing antigen
- SDS-PAGE (poly Acrylamide gel electrophoresis)
- Handling of nitrocellulose membrane
- Transfer of protein bands from gel to nitrocellulose membrane
Processing of nitrocellulose membrane for detection of antigen
- Preparation of sample material
- Preparation of nutrient media
- Plating the culture
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