DNA fingerprinting Technology
Molecular markers
Preparation of nucleic acid:
- Extraction of DNA
- Purification of DNA
-
- Gene analysis
- Genetic purity {Homozygosity &
homogeneity
- Genetic diversity
Gel Electrophoresis Technique:
- Agarose gel electrophoresis
- Native PAGE
- SDS – PAGE
- Ethidium bromide staining (PAGE/Agarose)
- Silver staining
Data Analysis:
- Analysis of resistant & susceptible
- lines analysis software
ELISA:
- Extraction of protein samples
- Processing of the samples for the
- detection of viral antigen.